Fibronectin CAS NO.86088-83-7

Product description :

Human fibronectin cell includes binding domain , heparin -binding domain and the CS1 site three functional areas. It consists of 574 amino acids with a molecular weight of 63kDa. It can greatly improve the efficiency of infection of mammalian cells, retroviral infection , and its principle is to : cell surface VLA-4 and VLA-5 , respectively, can be combined with the CS-1 domain site and a cell binding on Fibronectin , reverse viral vector may be combined with heparin binding domain . Fibronectin in the package container by the surface of culturing , the cells with the viral vector to the coexistence of high concentration , thereby enhancing the transfection efficiency of 30 to 70% . In addition Fibronectin can also stimulate lymphocytes to obtain the proliferation of thousands of times .

Product Features :

The retrovirus can be efficiently transferred to the gene of hematopoietic stem cells.

Avoid cytotoxicity due to the use of polybrene infection caused .

The same can stimulate the proliferation of lymphocytes obtained thousands of times the normal applicable infected cell lines .

You can get thousands of times to stimulate lymphocyte proliferation .

 Product Form : Lyophilized powder

Weight: 62,613 Dalton

Purity : SDS capillary electrophoresis > 97%

 Coating : The Fibronectin with PBS buffer was diluted to a packing 20 ~ 100 μg / ml after the coated plates (non-tissue culture treated), the coating fluid 4 ~ 20 μg/cm2.

 Precautions

1 Avoid repeated freezing and thawing .

2 When dissolved mix gently , do not use a vortex

 

Use: research use only and can not be used in clinical

 

Junsen Biotechnology Co., Ltd.

Contact: James Woo